Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions
In this test, the substrate treated with an immobilized antimicrobial is exposed to a bacterial suspension for one hour. After exposure, the suspension is tested to determine the reduction in the bacterial population over the contact time.
- This test is intended for use with materials treated with immobilized antimicrobial agents. Non-immobilized antimicrobials can leach from the surface into the test solution and may compromise test results.
- To determine if an antimicrobial treatment is leaching from the surface, leaching studies found in this method can also be performed.
- Testing can be performed with or without the leaching studies.
- Suitable sample substrates for this test include fabric, paper, powder or granular material, or solids with a treated surface; such as plastics, glass beads or chips, ceramics, metal chips.
- ASTM E2149-13A lists Escherichia coli ATCC#25922 as the test organism.