Fungal and Bacterial Tests - ISO 846 Methods
Plastics – Evaluation of the action of microorganisms
This method has 4 methods for determining the deterioration of plastics due to the action of fungi, bacteria, and soil microorganisms by visual examination, changes in mass, and/or changes in other physical properties.
Method A: Fungal Growth Test
Method B: Determination of Fungistatic Effects
Method C: Resistance to Bacteria
Samples are inoculated and incubated on an incomplete medium without a carbon source. If there is no growth in the agar surrounding the specimen, then the specimen does not contain any nutritive components.
Method D: Resistance to Microbially Active Soil (soil-burial test)
Samples are completely buried in natural soil with a known water-holding capacity and a specified moisture content. The soil-burial test has been included in this document because many plastics are used in permanent contact with soil and exposed to high humidity.